in vitro experiments on primary immune cells (lymphocytes, monocytes, neutrophils) from blood and immune organs (spline, ganglions) - in order to study the systemic immune response
in vitro experiments on human and porcine cells lines (epithelial cells: IPEC-1, Caco2, LLCPK, etc.) - in order to study intestinal barrier functions and local immune response
molecular biology assays - evaluation of genes expression of various biomarkers that are relevant for immune response and physiology of cells and tissues (blood, organs, muscles, milk, etc.): cytokines, enzymes, signalling molecules, various proteins and receptors, etc., in order to elucidate the metabolic pathways and influence of various factors (feed, environment) on animal production and health, etc.
proteomic assays (ELISA, western blot, electrophoresys, etc.) in order to study the protein profile in blood, cells & tissues (various applications relevant to animal health and production).
phytochemistry assays: assessing the bioactive compounds (e.g. polyphenols) from plant/plant extracts / feeds to evaluate their antioxidant capacity and activation/inhibition of various metabolic processes, etc.
in vivo trials in the main species and categories of farm animals to assess the effect of feed ingredients (special novel feed ingredients) or feed contaminants on the animal performances, health and immune response: