ANIMAL BIOLOGY LABORATORY



Imuno-nutrition studies

  • in vitro experiments on primary immune cells (lymphocytes, monocytes, neutrophils) from blood and immune organs (spline, ganglions) - in order to study the systemic immune response
  • in vitro experiments on human and porcine cells lines (epithelial cells: IPEC-1, Caco2, LLCPK, etc.) - in order to study intestinal barrier functions and local immune response
  • molecular biology assays - evaluation of genes expression of various biomarkers that are relevant for immune response and physiology of cells and tissues (blood, organs, muscles, milk, etc.): cytokines, enzymes, signalling molecules, various proteins and receptors, etc., in order to elucidate the metabolic pathways and influence of various factors (feed, environment) on animal production and health, etc.
  • proteomic assays (ELISA, western blot, electrophoresys, etc.) in order to study the protein profile in blood, cells & tissues (various applications relevant to animal health and production).
  • phytochemistry assays: assessing the bioactive compounds (e.g. polyphenols) from plant/plant extracts / feeds to evaluate their antioxidant capacity and activation/inhibition of various metabolic processes, etc.
  • in vivo trials in the main species and categories of farm animals to assess the effect of feed ingredients (special novel feed ingredients) or feed contaminants on the animal performances, health and immune response:
  • biochemistry analyses of blood parameters (albumins, bilirubins, minerals, glucose, cholesterol, triglycerides, IgA, IgM, IgG, creatinine, TGO, TGP, alkaline phosphatase, LHD, gamma GT, proteins, urea, etc.);
  • real time PCR analyses of genes expression of various biomarkers that are relevant for immune response from blood and tissues after animal slaughtering.

Animal genetic resources studies

  • Genetic markers for assisted selection of local breed
  • Taxonomy of local breed
  • Studies of quantitative genetics of the animal populations
  • Conservation methods for the animal endangered populations

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LABORATORY OF CHEMISTRY AND NUTRITION PHYSIOLOGY