LABORATORY OF BIOTECHNOLOGY

Research directions

  • Isolation of bacterial strains from intestinal microflora and phenotypical identification (Lactobacillus, Enterococcus, Bacillus, etc.) with the view of using them to produce feed additives.
  • Determining the optimal conditions for strain cultivation under laboratory conditions and production of pure cultures.
  • Testing the bacterial cultures for the synthesis of organic acids (lactic, phenyl-lactic, hydroxyphenyl-lactic, acetic and propionic) and enzymes (amylases, cellulases, proteases, etc);.
  • Experimental administration of the strains in monogastric animals feeding and studies regarding their influence on the intestinal bacterial microflora.
  • Experimental use of the strains to ensile corn and alfalfa.
  • Improving the existing technologies and development of new technologies for the production of feed additives with growth promoting role to be used in animal feeding and with role of preserving the feeding value of the forages.
  • Determining the effects of the probiotic products and preservatives used as biological alternative in pig and poultry feeding and to ensile forages.
  • Studies on the intestinal microflora or animals correlated with the nutrition and immunity of the host animals.
  • Studies on the alleviation of the excreted intestinal bacterial microflora influence on the environment.


Results – Laboratory of Biotechnology

  • Identification and phenotypical characterization of strains from the following species: Lactobacillus acidophilus, L. fermentum, L. paracasei; L. plantarum, Enterococcus faecium, Bacillus amyloliquefaciens, etc.
  • Establishment and maintenance of IBNA collection of bacterial strains.
  • Monovalent or polyvalent cultures of Lactobacillus acidophilus, L. paracasei; L. plantarum, Enterococcus faecium tested for growth promoting activity on broilers and on piglets during the weaning crisis.
  • Beneficial impact of the monovalent cultures of probiotics to balance the intestinal microflora on the broilers and of the piglets during the weaning crisis.
  • Positive impact of the monovalent cultures of probiotics to alleviate the environmental microbiological pollution of the rearing halls.
  • Quantification of the enzymatic activity of lactic bacteria strains: synthesis of alpha-amylase (Lactobacillus acidophilus) and cellulase (L. plantarum).


Other activities / services

  • Bacterial and mycological examinations of feed ingredients, compound feeds, protein-vitamin-mineral concentrates and biological samples.
 

 

CS III

Personal auxiliar

 

BULGARU Daniela

 

 

Muncitor

Infrastructure

 

 

 

The laboratory has the functional capacity to isolate, identify, select and replicate strains of lactic acid bacteria, yeasts, etc., and to produce biological preparations used to preserve the feeding value of the forages and with probiotic role in animal feeding.

 

 

For these activities, the laboratory uses autoclaves, ovens, thermostats, pH-metre, microscopes, fume chamber for bacteriological purposes, centrifuges, refrigerators, scales, magnetic stirrer, automatic reader/counter of colonies, culture media dozing device, water baths, API 50CH and API Strep (Biomerieux) kits, to identify Lactobacillus spp., Bacillus spp., Enterococcus /Streptococcus spp., selective culture mediums, Gramm colouring kit, etc.




 

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LABORATORY OF ANIMAL NUTRITION